Molecular Biologist / Microbiologist

Kimberly M. Moss

E-mail:kmmoss75@gmail.com

 

EDUCATION 

Bachelor of Science, Biology                                                                               

Kennesaw State University
(KSU)   Kennesaw, GA             
                         

Graduation date: May 2005           

Related Courses:
Microbiology, Microbiology Lab, Medical Genetics, Biochemistry, Organic
Chemistry 1 & 2, Cell & Molecular Biology, Medical Microbiology,
Genetics, Human Physiology, Developmental Biology.



EXPERIENCE

Georgia Public Health Laboratory (GPHL)                                

1749 Clairmont Rd Decatur,
GA 3003

Department of Public Health, Emergency
Preparedness Bioterrorism Unit, Molecular Biology Unit

Medical Clinical Laboratory Specialist                                                                            August 2011-Present

The Georgia Department of Public Health (DPH)
is the lead agency entrusted by the people of the State of Georgia with the ultimate
responsibility for the health of communities and the entire population. At the
state level, DPH is divided into numerous branches, sections, programs and
offices and at the local level, DPH functions via 18 health districts and 159
county health departments. The mission of the Georgia Department of Public
Health (DPH), Georgia Public Health Laboratory (GPHL) is to improve the health
status of Georgians by providing accurate, timely and confidential clinical and
non-clinical laboratory testing in support of Division of Public Health
programs, activities and initiatives as well as performing tests for Emergency
Preparedness.

Biological Threat / Molecular Biology Unit

  • Directly supports GPHL emergency response program, regularly requiring 24 hour on-call duty with obligation to travel as needed for training and emergency responses and surge support. Work normal shifts, weekend shifts, holiday shifts, and any hours deemed necessary in the event of public health emergency. Emergency respondent in the situation of a suspected bioterrorism event, report results via LRN results messenger. Follow, document, process, & triage samples while maintaining chain-of-custody for specimens  received in white powder FBI /GBI events.
  • Mandatory compliance with the Patriot Act of 2001 with requirements related to the Select Agent (SA) program, including FBI/CJIS security risk assessment.
  • Performs biosafety techniques with proper PPE in BSL3 and BSL2 with BSC 2 while utilizing sterile techniques.
  • Analytical position, performing a wide range of laboratory test defined as high complexity by the 1988 Clinical Laboratory Improvement Amendment (CLIA) in order to obtain data for the use in medical diagnosis and treatment.
  • Perform protocols and validate reagents for Association of Public Health Laboratories (APHL) defined protocols to monitor the population of Georgia for suspected outbreaks in the interest of public health. Implements and follows Quality Assurance Plans and Quality Control procedures to ensure accurate results.
  • Analyzes specimens, interprets and records results, and reports findings via LIMS: APOLLO, LRN Results Messenger, and notifying appropriate state or federal epidemiologist, coordinator, or health facility.
  • Perform laboratory support activities such as maintaining equipment, databases, maintaining lab cost, ordering lab supplies, tracking reagent inventory and other administrative aspects of laboratory functions.
  • Communicates directly with State Epidemiologist on Outbreak Surveillance, Specimen Collection, and Diagnostic Testing of Norovirus and Influenza A & B, along with Conformational Referral of specimens to CDC for additional testing, particularly novel or resistant influenza strains.
  • Evaluate laboratory performance of the assays with extensive analysis of assay characteristics, sensitivity and specificity; evaluate new application software technologies for laboratory instruments. Testing reagents to ensure lot quality control.
  • In charge of processing, preparing and shipping reference aliquot sets of various specimen types samples to CDC, following regulations: 42 CFR Part 72 – Select Agent Rule and DOT & IATA .
  • Participate in CDC Proficiency Tests (PT), as well as CAP, CLIA, State of Georgia lab inspections. Update and create SOP
    and protocols for Molecular Biology Unit.
  • Isolation of RNA and DNA via Qiagen kits, manual preps, and cell lysate techniques.
  • Performs and validates CDC & Laboratory Response Network (LRN) defined protocols in a designated BSL3 laboratory for the detection of select agents as defined by the CDC and the FBI.
  •  Molecular techniques used to id rash illness, microbial, viral, and toxins classified as select agents.
  • Screen viral samples for the novel influenza virus variants as well as the
     seasonal subtypes using rRT-PCR.
  • Pyrosequencing  for Influenza A specimens to determine and track anti-viral resistance.
  • Capillary based sequencing of the 16s DNA region of unidentifiable or inconclusive special bacteriology
    samples through conventional microbiology methods, to help determine the genus
    and species of microbes. BLAST sequence into National Center for Biotechnology
    Information (NCBI) database for microbial reference id.
  • Analysis of PCR products via 2% gel and E-gel UV visualization of amplicons.
  • Food Emergency Response Network (FERN)/ FDA procedures for isolation and identification of Bacillus anthracis, Clostridium botulinum Toxin, Francisella tularensis, and Yersnia Pestis in food samples in response to emergencies involving biological, chemical, or radiological contamination of food.

 

  • Instruments used: Applied Biosystems 7500 Fast DX, 3130 xl, and GeneAmp 9700, Roche MagNA Pure LC, MagNA Pure Compact, Roche Lightcycler 1.2, Qiagen PyroMark Q24.

Chemical Threat Unit

 

 

Instrumentation:

 

 

 

-LC/MS/MS System Applied Biosystem MDS ScieX API4000

- HPLC Agilent Technologies 1200 Series

 - Rapid Trace SPE Workstation

 - TurboVap LV Concentration Workstation

 -Supelco Visiprep 24th DL (Manual Extraction)

    Methods:

      - Ricinine and L-Abrine by
      LC/MS/MS: Rapid method for identification of toxic agent to assess degree of
      exposure. Column: ABRN-phenomenex-synergl 2.5u Poker RP (100 X 2.00mm)

      - Metabolic Toxin Panel
      (MTP) by LC-MS/MS: Negative electrospray ionization rapid method for
      identification of toxic agent to assess degree of exposure. Column: MTP Sielc
      Primesep B2 (2.1 X 50 mm).

      - Organophosphate Nerve Agent
      (OPNA) Metabolites by LC/MS/MS: Negative electrospray ionization method used
      for the analysis of metabolites of 5 common organophosphorous nerve agents:
      sarin (GB acid - methylphosphonic acid, isopropyl ester), soman (GD acid  -methylphosphonic acid,1,2,2-trimethylpropyl
      ester),  cyclohexylsarin (GF acid –
      methyl phosphonic acid, monocyclohexyl ester) Russian VX (rVX acid – methyl
      phosphonic acid, 2-methylpropyl ester) and VX acid (methylphosphonic acid,
      monoethyl ester) and identification of toxic agent to assess degree of
      exposure. Column: OPNA Atlantis columns HILIC Silica 3um (2.1 X 50mm).

       

      • Sample preparation of nerve agents, Ricinine/Abrine and Metabolic Toxins.
      • HPLC Setup,including mobile phase and column setup, preventative maintenance, cleaning
        source, changing vacuum oil, and replacing needle and needle seat.
      • HPLC tuning and troubleshooting.
      • HPLC Agilent Analyst software analysis including equilibration, batch setup, sample analysis, data
        analysis, and resulting.
      • Surge capabilities for Chemical threat (ICP/GC/LC/MS)



      Emory University School
      of Medicine                                 
      2165 North Decatur
      Rd Decatur, GA 30033

      Department of Human Genetics, Clinical Molecular
      Genetics Laboratory
       

      Clinical Genetic Technologist                                                             

       February 2011-August 2011


      Emory Genetics Laboratory (EGL) is apart of the division of Human Genetics of Emory
      University School of Medicine. They are a not for profit clinical testing lab,
      with a focus on rare genetic human disorders. EGL offers a complete
      comprehensive testing facility, including Biochemical, Cytogenetics, and
      Molecular Genetics clinical testing. Additionally, they offer a Clinical
      Genetics Clinic staffed with Genetic Counselors, Dieticians, Nurses, and
      Physicians to serve our patients and their families.

      • Technologist for Human Molecular Diagnostic Rare Genetic Disorders.  
      • Allele specific testing and Luminex testing for Ashkenazi Jewish panel, Cystic Fibrosis Newborn Screening, AlphaThalassemia.
      • Performed PCR amplification and fragment size analysis of myotonic dystrophy syndrome to detect smaller alleles, combined with Southern Blot analysis to detect large expansions.
      • Quantitative methylation sensitive PCR analysis of the SNRPN gene on Prader-Willi and Angelman Sydromes.
      • Testing for spinal muscular atrophy via PCR amplification of exons 7 and 8 of the SMN1 gene. Homozygous deletions of either or both exons detected using restriction enzyme fragment size analysis.
      • Chromosomal abnormalities and FMR1-related Premature Ovarian Failure (POF) and Maternal Cell Contamination (MCC) detected by PCR amplification and fragment analysis. Southern blot analysis with methylation sensitive enzymes performed to detect expanded alleles and methylation status of the FMR1 gene.
      • Regular programming, set-up, calibration, maintenance, and trouble shooting of
      • Molecular Instrumentation: Thermocyclers AB GeneAmp 9700, 3730 Analyzer (Applied Biosystems), ABI
             3100/3730xL, and AlphaImager 2000 (UV Gel Visualization).

      Georgia Public Health Laboratory (GPHL)                       

      1749 Clairmont Rd Decatur,
      GA 30033

      Department of Public Health, Emergency
      Preparedness Bioterrorism Unit, Molecular Biology Unit

      Medical Clinical Laboratory Specialist (Contractor)                                        

       February 2010 – June 2010                                                                                                                        

       

      • Communicates directly with State Epidemiologist on Outbreak Surveillance, Specimen Collection, and Diagnostic Testing of Norovirus and Influenza A & B, along with Conformational Referral of specimens to CDC for additional testing, particularly novel or resistant influenza strains.
      • Molecular techniques used to id Influenza Strains.
      • Screen viral samples for the novel influenza virus variants as well as the seasonal subtypes using rRT-PCR.
      • Updated Influenza SOP for extraction and PCR methods.

      MolecularBiology Laboratory Skills & Techniques

      • Aseptic Technique 

      • Southern Blotting

      • UV gel reading

      • Restriction digestion
      • Gel Electrophoresis
      • rRT-PCR, RT-PCR
      • Multiplex PCR
      • Pyrosequencing
      • Capillary Based Sequencing
      • In-situ hybridization
      • SNPs analysis
      • DNA/RNA nucleic acid extraction, isolation,
        purification



      CryoLife, Inc

      1655 Roberts Blvd, NW Kennesaw,
      GA 30144

      Quality Assurance & Regulatory Affairs

      Microbiology Technician II                                                                                  

      October 2007-November 2009

      A Biomedical Company providing preservation of human tissues for cardiac and vascular reconstructive surgeries.

      • Performed microbiological monitoring of processing areas in all departments, including airborne particles and water testing for microbes, conductivity, endotoxin, and total organic carbon.
      • Tested human heart and vein tissue samples via membrane filtration for sterility following standard operating procedures for aseptic techniques to avoid contamination of products to be used for implantation.
      • Responsible for performing corrective and preventive actions.
      • Performed QC/QA testing on all media used in the laboratory for sterility.
      • Maintained unit autoclave by weekly testing of leak test and keeping track of recordings of use for audits by the FDA.
      • Updated SOP for the cleaning and maintenance of the VITEK 2 system used for rapid microbial identification and antibiotic susceptibility testing.
      • Used the BacT/ALERT system for microbial detection used to conduct sterility testing.

      MicrobiologyLaboratory Skills & Techniques

      • Aseptic Transfer
      • Staining Techniques: Gram, Acid-Fast
      • Pipetting
      • Serial Dilutions
      • Streak and spread plate
      • Tube Biochemical test
      • Microbial ID
      • Microscopic Examination for morphology
      • Colony Counts
      • Quadrant streaking for isolation
      • Inoculation and incubation
      • Wet Mount
      • Serology

      TRAINING, CERTIFICATIONS, & PROFESSIONAL ORGANIZATION
      MEMBERSHIPS
      • CDC/LRN Rapid Methods Training (Time-Resolved Fluorescence-TRF) 09-2011 in St. Paul, Minnesota 
      • Association of Public Health Laboratories (APHL) Member


      COMPUTER EXPERIENCE, COMPETENCIES & SKILLS

      • Microsoft Outlook, Word, Excel, PowerPoint, Office Document Imaging and Scanning, Internet. 
      • Highly skilled using instrument application software, sample set-up, instrument calibration, and maintenance. 
      • Laboratory Information Management Systems (LIMS): APOLLO/MLAB, LRN Results Messenger, Medgis, Lotus, MISYS, Horizon Clinicals, Star Navigator. 
      • Protocol, SOP, Quality Assurance plan per review and editing experience. 
      • Excellent team member with great independent work habits. 

      • ID#: 72238
      • Location: Alpharetta, GA , 30004

      Don't Be Fooled

      • When selling, do not put your home address in your ad.
      • To avoid scams, buy and sell with people you can meet locally, in person.
      • When meeting with someone you don't know, meet in a public place. If that's not possible, have a buddy with you. Also, carry a cell phone; if you feel unsafe, you can call a trusted friend, and stay on the line.
      • Never give out financial or private information like account numbers, PayPal login, or social security number.
      • If an offer sounds too good to be true, it is. Walk away!